The cytokine ELISA Kits enable researchers to measure specific cytokines in biological samples, such as plasma, serum and tissue culture supernatants. This can give valuable insight into changes that occur with various diseases. Interleukins, interferons and members of superfamilies TGF-beta and tumor necrosis factors are some of the cytokines that are of particular interest.
The two-site sandwich ELISA principle is used in these assays. Two highly specific antibodies are used for detection of a target analyte. Multiple steps are taken during development to ensure that cytokine ELISA equipment provides superior performance without the necessity of further assay optimization.
Cytokine Kits are the preferred choice for life scientists because they have undergone rigorous validation testing. Cytokines are a cornerstone of any study that deals with inflammation, whether it is an in vitro cell culture system or an in vivo animal model.
The cytokine profile as a whole and the relative abundance of one cytokine, and the endogenous inhibitors, define an inflammatory process that is in motion. The cytokine profile can be used to identify the source of an injury, insult, or infection and to help stage the disease process.
These studies focus on the ability to identify, quantify, and distinguish a single cytokine among a variety of biomolecules in a sample. The indirect sandwich enzyme-linked immunosorbent assay (ELISA) is one such method. Cytokine assays assess the preparation and treatment for blood and tissue samples that are related to cytokines.
Because cytokines play a pivotal role in the progression and regression of many diseases and are biomarkers for many others, cytokine assays are crucial in disease diagnosis and monitoring. Cytokines assays can be described as a serious of methods and protocols.